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Roles of Gibberellin and Auxin in Promoting Seed Germination and Seedling Vigor in Pinus massoniana

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Abstract:

Masson pine (Pinus massoniana Lamb.) is an important timber species and is widely planted in southern China. A large quantity of seed is required for sowing each year. However, low germination in the nursery has resulted in low seedling yields with a consequent negative impact on the Masson pine afforestation program. The purpose of our study was to determine whether gibberellin (GA3) or indole-3-acetic acid (IAA) would be more effective in propagating the species from seeds for afforestation and to elucidate their effects on seed respiration and endogenous hormone levels. Seeds were soaked in GA3 and IAA solutions of different concentrations at 25° C in the dark for 24 hours, and a germination test was conducted. Seed respiration was measured indirectly by estimating oxygen consumption in a closed system. Endogenous hormone levels were analyzed by gas chromatography/mass spectrometry (GC/MS). The germination test showed that the most significant promotion of seed germination and seedling vigor occurred at 5 × 10−5 M GA3 in both varieties and that the effects of IAA were variety dependent. Oxygen-sensing measurements showed that increased metabolism time, critical oxygen pressure, and relative germination time were decreased and the oxygen metabolism rate was increased in IAA- and GA3-treated seeds. GC/MS analysis showed that the application of 5 × 10−5 M GA3 decreased the endogenous abscisic acid (ABA) content, but increased IAA and GA1 levels. Our experiment suggests that exogenous GA3 improves seed germination and seedling vigor of Masson pine through promoting seed respiration or lowering the ABA level and stimulating IAA and GA1 biosynthesis.

Keywords: Pinus massoniana; auxin; gibberellin; oxygen-sensing; seed vigor

Document Type: Research Article

DOI: https://doi.org/10.5849/forsci.12-143

Publication date: 2014-04-01

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  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Average time from submission to first decision: 62.5 days*
    June 1, 2016 to Feb. 28, 2017

    Also published by SAF:
    Journal of Forestry
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