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The Effects of GA3 and Organic Solvents on Acclimatization of Tissue Culture Propagated Black Cherry (Prunus serotina Ehrh.) Plantlets

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Both gibberellic acid (GA3) and chilling were effective in inducing stem elongation of black cherry plantlets during acclimatization. At the rate of 100 mg/l, a foliar spray of GA3 was effective in stimulating stem elongation, but the effect was temporary. Most plantlets elongated to approximately 9 cm, but then set a resting bud. At rates above 200 mg/l, GA3 severely stunted root growth. An organic solvent solution enhanced the stem elongation response to GA3. When plantlets were treated with 100 mg/l GA3 in 0.2% DMSO and 54% ethanol, reinitiation of stem elongation was quicker, and bud set was delayed. Although the combination of GA3 and organic solvents induced stem elongation, it did not fully substitute for a chilling period. The minimum chilling period required to induce terminal bud break was 6 wk, and an additional 4 to 6 wk induced more rapid terminal bud break and stem elongation. For. Sci. 39(4): 644-654.

Keywords: DMSO; Micropropagation; clonal propagation; gibberellic acid

Document Type: Journal Article

Affiliations: Faculty of Forestry, State University of New York, Syracuse, New York

Publication date: 1993-11-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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