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Notes: Amyloglucosidase and α-Amylase Enzymes for Determining Starch Concentration in Ponderosa Pine Root Tissue

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Purified combinations of α-amylase and amyloglucosidase were compared with combinations direct from the supplier to determine their relative precision in estimating starch concentration in root tissue of ponderosa pine (Pinus ponderosa Laws.). For two of the combinations, estimates of starch by an enzymatic digest method were nearly identical, suggesting that an insignificant amount of contaminants was present in the unpurified enzyme combination. The third combination tended to yield slightly lower values of starch, yet was still similar in precision to the other two. We concluded that the purity of purchased enzymes was sufficient for use in the enzyme digest method. For. Sci. 37(5):1479-1483.

Keywords: Enzyme activity; amylopectin; amylose; enzymatic hydrolysis; seedling vigor

Document Type: Miscellaneous

Affiliations: Assistant Professor, Department of Forest Science, Oregon State University, Corvallis, OR 97331

Publication date: November 1, 1991

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