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Notes: In Vitro Responses of Conifer Adventitious Shoots and Calli Inoculated with Gremmeniella abietina

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Larix decidua adventitious shoots were inoculated with axenic vegetative hyphae and conidia of Gremmeniella abietina. Dense host surface mycelial growth and needle necrosis occurred after inoculation with vegetative hyphae and conidia within two weeks and four weeks, respectively. The pathogen completed its life cycle by producing pink conidial pustules on some infected propagules within one month. Light and scanning electron microscopic examination showed that hyphae penetrated commonly through or between epidermal cells and rarely through the stomates. Differential responses were evident within and between shoot clones. Inoculated calli of resistant L. leptolepis and Abies balsamea showed less mycelial growth than did L. decidua calli. Inoculation success was a function of spore inoculum density. In vitro responses correlated well with known in vivo responses. Our rapid (30-d) in vitro resolution of host resistance suggests that these methods may reduce the time required for evaluating relative disease resistance as compared to traditional field tests. For. Sci. 33(4):1047-1053.

Keywords: Disease resistance screening; scleroderris canker

Document Type: Miscellaneous

Affiliations: BioSource Forest Biotechnology Group, Michigan Technological University, Houghton, MI 49931

Publication date: 1987-12-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Average time from submission to first decision: 62.5 days*
    June 1, 2016 to Feb. 28, 2017

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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