Notes: Fertility of Douglas-fir Pollen After One Year of Storage in Liquid Nitrogen
Author: Copes, Donald L.
Source: Forest Science, Volume 31, Number 3, 1 September 1985 , pp. 569-574(6)
Publisher: Society of American Foresters
Abstract:Results indicated that Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) pollen stored in liquid nitrogen (- 196°C) for 1 year was nearly as fertile as fresh pollen when used for controlled pollinations. Percent of filled seed was 79.9 for liquid nitrogen-stored pollen and 81.9 percent for fresh pollen of the current year. Filled seeds per cone averaged 46.3 and 47.9 for liquid nitrogen-stored pollen and fresh pollen, respectively. Flowers pollinated with fresh or stored pollen experienced significantly greater levels of flower abortion than flowers that were isolated but not pollinated; survival of flowers was 45.3, 64.5, and 93.8 percent, respectively. Methods used in cryogenic storage with liquid nitrogen are simple and enable tree improvement workers and forest geneticists to effectively store Douglas-fir pollen for at least 1 year without significant loss of fertility. Forest Sci. 31:569-574.
Document Type: Miscellaneous
Affiliations: Principal Plant Geneticist, U.S. Department of Agriculture, Forest Service, Pacific Northwest Forest and Range Experiment Station, Forestry Sciences Laboratory, Corvalis, OR 97331
Publication date: September 1, 1985
- Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
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