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Tissue Culture of Propagation of Mature Trees of Prunus serotina Ehrh. I. Establishment, Multiplication, and Rooting in Vitro

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Bud tissue explants were obtained from winter buds of grafted Prunus serotina Ehrh. trees growing in a seed orchard in Pennsylvania and from three 50-year-old P. serotina trees growing in central New York. Twigs were collected and explants prepared in late winter just as the buds were beginning to swell. Explants were established and elongated on Murashige and Skoog inorganic salt medium (MS), supplemented with 100 mg/l I-inositol, 0.4 mg/l thiamine-HCl, 1.0 mg/1 N-6-benzylaminopurine (BAP), 0.1 mg/l gibberellic acid (GA3), 0.1 mg/l indole-3-butyric acid (IBA), 2 percent sucrose, and 0.7 percent phytagar. Multiple shoots were induced on the same medium but with 0.75 mg/l BAP, 0.2 mg/l GA3, 0.01 mg/l IBA, and 3 percent sucrose. A five-fold shoot multiplication rate was obtained every 3 to 4 weeks. Root formation was induced in vitro after shoots had grown for 14 days on MS medium supplemented with 100 mg/l I-inositol, 0.4 mg/l thiamine-HCl, 1.0 mg/l IBA, 3 percent sucrose, 0.7 percent phytagar on a 16-hour photoperiod. Rooting could be enhanced by incubating cultures in continuous darkness. The addition of rutin or quercetin to the rooting medium increased rooting in the light. Plantlets were successfully transferred to soil, following establishment, multiplication, and rooting in vitro. Forest Sci. 31:201-208.

Keywords: Black cherry; clonal propagation; micropropagation

Document Type: Journal Article

Affiliations: Assistant Professor, School of Forestry, College of Environmental Science and Forestry, SUNY, Syracuse, NY 13210

Publication date: March 1, 1985

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
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