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Notes: The Determination of Percentages of Living Tissue in Woody Fine Root Samples Using Triphenyltetrazolium Chloride

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Knievel (1973) developed a method for evaluating the ratio of live root material to dead based on the relationship between that ratio and triphenyltetrazolium chloride (TTC) reduction per gram of root tissue. The method allows the processing of quantities of roots in bulk but has only been tested on white, nonwoody roots. A procedure very similar to Knievel's was tested on woody roots obtained from a mature white oak (Quercus alba L.) stand. Extractants from live and from dead roots both produced stains whose spectrophotometric absorbance at 480 nm correlated highly with the dry weight of tissue; slopes were distinctly different, however, with the live tissue producing a darker, redder extract. By solving regression equations for live and dead tissue simultaneously, it was possible to compute the ratio of live to dead tissue. Forest Sci. 30:965-970.

Keywords: Quercus alba; TTC; fine roots; root mortality; root turnover

Document Type: Miscellaneous

Affiliations: Professor, School of Forestry, Fisheries, and Wildlife, 1-31 Agriculture Building, University of Missouri, Columbia, Mo. 65211

Publication date: 1984-12-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Average time from submission to first decision: 62.5 days*
    June 1, 2016 to Feb. 28, 2017

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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