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Development of Pisolithus tinctorius Ectomycorrhizae on Pine Seedlings Using Basidiospore-Encapsulated Seeds

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Procedures for encapsulating seed of various pine species with basidiospores of Pisolithus tinctorius were developed. In container tests in 1979 with Pinus taeda, P. virginiana, P. elliottii var. elliottii, P. echinata, P. oocarpa, and P. caribaea, seed encapsulation with spores increased seed diameter by 1.5 mm and decreased seedling establishment by up to 60 percent. Only moderate amounts of Pisolithus ectomycorrhizae developed on most tree species. However, abundant P. tinctorius ectomycorrhizae were developed in 1980 on P. taeda seedlings in both containers and in microplots by using a coating that increased seed diameters by only 1 mm and by adjusting the watering schedule. Encapsulation with 1 or 2 mg of spores per seed reduced seedling establishment by 20 percent in microplots; 1 mg spores per seed did not reduce seedling establishment in containers. One mg of spores (1.1 x 106 spores) per seed was as effective as 2 mg of spores per seed in forming Pisolithus ectomycorrhizae. Dressing encapsulated seeds with thiram, captan, or benomyl had no detrimental effect on seedling establishment or P. tinctorius ectomycorrhizal development on P. taeda seedlings in microplots. Captan or thiram, however, increased and benomyl decreased Pisolithus ectomycorrhizal development in containers. Thiram or benomyl also decreased seedling establishment in containers. Encapsulated seeds should be watered daily for 1 week after sowing and on alternate days for the next 2 weeks. This watering regime aids seed germination and disintegration of the pelletizing matrix to release the spores. Expanded tests on the significance of spore encapsulated seed in conventional nurseries and direct seeding with various tree species are now warranted. Forest Sci. 30:897-907.

Keywords: Pinus taeda; container-grown seedlings

Document Type: Journal Article

Affiliations: Operations Manager, International Forest Seed Company, Box 290, Odenville, AL

Publication date: 1984-12-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Average time from submission to first decision: 62.5 days*
    June 1, 2016 to Feb. 28, 2017

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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