Notes: Carbohydrate and Nitrogen Concentrations in Leaves of Three Shrub Species Following Microwave, Autoclave, and Air-Drying Treatments

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Abstract:

Microwave treatment compares favorably with conventional autoclave treatment for preparing leaf samples of Purshia tridentata, Atriplex canescerts, and Artemisia tridentata for analyses of total nonstructural carbohydrates (TNC) and for analyses of total organic N (TN). Contrary to previous literature, air-drying was also an acceptable procedure for preparing P. tridentata leaves for TNC analyses. For measurements of TN, microwave, autoclave, and airdrying treatment of leaf tissue gave the same results. Results of the air-drying treatment have important implications for sampling shrub species in remote field research locations where autoclave and microwave facilities are not available. Forest Sci. 30:113-116.

Keywords: Artemisia tridentata; Atriplex canescens; Purshia tridentata; Total nonstructural carbyhydrates (TNC); autoclave treatment; plant sample preparation; total nitrogen (TN)

Document Type: Miscellaneous

Affiliations: Soil Scientist, USDA Soil Conservation Service, Walla Walla, WA 99362

Publication date: March 1, 1984

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