Genetic variation in chemical, physiological, and anatomical traits associated with the oleoresin physiology of Scotch pine was studied in a rangewide provenance plantation. Fifty seedlots representing 18 varieties were examined. The resin acids showed strong positive correlations with each other. Nine of thirteen resin acids and total resin acids showed genetic variation, with seed sources from Scandinavia having the highest concentrations and those from central Europe the lowest. Russian and Siberian varieties had the highest water potential while the Scandinavian varieties had the lowest oleoresin pressure and the smallest resin canal cross-sectional areas. No variation was observed in the density of xylem resin canals. The resin acids as a group show evidence of pleiotropy, but all of the physical traits were genetically independent of each other. Forest Sci. 28:582-589.