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Tissue Culture Plantlets Produced From Pinus monticola Embryonic Materials

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Methodology for tissue culture propagation of clones from western white pine, Pinus monticola, embryonic materials is presented, and the efficiency of clone production is discussed. Bud initiations from excised cotyledons yielded 17.6 buds per clone, whereas initiations from whole embryos yielded 7.4 buds per clone. Regardless of the initiation method, about 19 percent of the buds initiated eventually elongated to a height of 5 mm or more. Currently, 30 percent of these can be rooted. Forest Sci. 27:299-304.

Keywords: Adventitious buds; clone production efficiency; conifer culture; propagule rooting

Document Type: Journal Article

Affiliations: Visiting Assistant Professor, Department of Botany, North Carolina State University, Raleigh, NC 27650

Publication date: June 1, 1981

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