Scanning Electron Microscopy of Shoot and Root Development in Sweetgum Callus Tissue Culture
Abstract:Buds and roots differentiated on callus cultures of sweetgum (Liquidambar styraciflua) were studied by scanning electron microscopy. Stomata are formed early and a cuticle is deposited over the surface indicating the early differentiation of an active epidermal layer. Young, overwintering leaves contained in the spring buds of trees do not differentiate stomata and cuticle until the bud starts to enlarge and the tips of the leaves are about to break through the bud scales. Typical roots and rootlike structures are also differentiated in callus culture. The more typical roots have a well-defined apical zone with a root cap, zone of enlargement, and root hairs. Other radially symmetrical outgrowths although having the form of roots neither have an external epidermal layer nor normal root hair formation. Forest Sci. 27:206-212.
Document Type: Journal Article
Affiliations: Professor of Forest Resources, School of Forest Resources, University of Georgia, Athens, Georgia 30602
Publication date: March 1, 1981
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