Notes: Biological Control of Decay Fungi: A Wood Disk Evaluation Method

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Frozen, debarked, tree branch disks were surface-sterilized by dipping in boiling water and then placed in petri dishes containing water reservoir. One-half of the disk top surface was swabbed with a cell suspension of a potential decay fungus antagonist; 2 days later the treated and the nontreated halves were inoculated with plugs from an agar culture of a decay fungus. After 5-8 days, some antagonists prevented decay fungus mycelium from reaching the bottom of the disk. Certain bacteria, fungi, and yeasts, isolated from Norway maple wood on a Norway maple diffusate agar and tested with Norway maple disks, were effective against four decay pathogens of maple. Sound, surface-sterilized Norway maple disks were naturally inhabited by endophytic resident bacteria, some of which were effective antagonists in disk tests. Forest Sci. 24: 560-564.

Keywords: Acer platanoides; Bacteria; Fomes conatus; Fomes igniarius; Inonotus glomeratus; Norway maple; Oxyporus populinus; Phellinus igniarius; Pholiota adiposa; Polyporus glomeratus; endophytic resident

Document Type: Miscellaneous

Affiliations: Professor of Plant Pathology, The Ohio State University, and Ohio Agricultural Research and Development Center, Wooster, OH 44691

Publication date: December 1, 1978

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