Isoenzyme Activities Differ in Compatible and Incompatible Douglas-fir Graft Unions

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Abstract:

Unusually dark-stained peroxidase bands at Rm (relative mobility) 0.77 to 0.80 and esterase bands at Rm 0.50 to 0.56 were found in tissues of incompatible Douglas-fir graft unions. Ninety- to 100-percent accuracy in identifying incompatible grafts was achieved with union tissues 10 to 15 months after grafting (February to May). A practical graft testing program is possible using electrophoretic methods for detecting changes in stain intensity in bands from incompatible graft unions. Enzyme activity of bands with other Rm values was not correlated, or was only weakly correlated, with graft incompatibility. Enzyme similarity of stock and scion tissues could not be used to determine potential compatibility of stock-scion combinations before grafting. No correlation with graft incompatibility was found with enzyme activity or the presence or absence of isoenzyme bands of catalase, acid phosphatase, or leucine aminopeptidase. Forest Sci. 24:297-303.

Keywords: Vegetative propagation; pseudotsuga menziesii

Document Type: Journal Article

Affiliations: Principal Plant Geneticist, Forestry Sciences Laboratory, Pacific Northwest Forest and Range Experiment Station, USDA Forest Service, Corvallis, Oregon

Publication date: June 1, 1978

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