@article {Winton:1972:0015-749X:151,
	author = "Winton, Lawson L.",
	title = "Notes: Callus and Cell Cultures of Douglas-Fir",
	journal = "Forest Science",
	volume = "18",
	number = "2",
	year = "1972",
	abstract = "Cell suspensions of Douglas-fir doubled in volume weekly, in 300 ft-c of continuous tungsten-fluorescent light. The medium was adapted from Brown and Lawrence and contained 25 mg/liter IAA and 0.5 mg/liter kinetin. On the same medium solidified with agar, callus generally died after 1-2 monthly subcultures, but lived for an additional 1-2 passages when the tungsten light was omitted. The growth hormones were changed to 1-5 mg/liter &#946;-naphthoxyacetic acid and 0.0-1 mg/liter N<SUP>6</SUP>benzylaminopurine, and the callus was grown in 16 hr of fluorescent light at 25&#176;C, alternating with 8 hr of darkness at 15&#176;C. This has resulted in continuous growth, for over one year, of friable green callus in the light and firm white callus in the dark. Forest Sci. 18:151-154.",
	pages = "151-154",
	url = "http://www.ingentaconnect.com/content/saf/fs/1972/00000018/00000002/art00013",
	keyword = "Pseudotsuga menziesii, tissue culture, vegetative propagation"
}