Notes: Callus and Cell Cultures of Douglas-Fir
Abstract:Cell suspensions of Douglas-fir doubled in volume weekly, in 300 ft-c of continuous tungsten-fluorescent light. The medium was adapted from Brown and Lawrence and contained 25 mg/liter IAA and 0.5 mg/liter kinetin. On the same medium solidified with agar, callus generally died after 1-2 monthly subcultures, but lived for an additional 1-2 passages when the tungsten light was omitted. The growth hormones were changed to 1-5 mg/liter -naphthoxyacetic acid and 0.0-1 mg/liter N6benzylaminopurine, and the callus was grown in 16 hr of fluorescent light at 25°C, alternating with 8 hr of darkness at 15°C. This has resulted in continuous growth, for over one year, of friable green callus in the light and firm white callus in the dark. Forest Sci. 18:151-154.
Document Type: News
Affiliations: Research Associate in the Division of Natural Materials & Systems, and Associate Professor of Forest Biology, The Institute of Paper Chemistry, Appleton, Wisconsin 54911
Publication date: June 1, 1972
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