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Notes: Callus and Cell Cultures of Douglas-Fir

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Cell suspensions of Douglas-fir doubled in volume weekly, in 300 ft-c of continuous tungsten-fluorescent light. The medium was adapted from Brown and Lawrence and contained 25 mg/liter IAA and 0.5 mg/liter kinetin. On the same medium solidified with agar, callus generally died after 1-2 monthly subcultures, but lived for an additional 1-2 passages when the tungsten light was omitted. The growth hormones were changed to 1-5 mg/liter -naphthoxyacetic acid and 0.0-1 mg/liter N6benzylaminopurine, and the callus was grown in 16 hr of fluorescent light at 25°C, alternating with 8 hr of darkness at 15°C. This has resulted in continuous growth, for over one year, of friable green callus in the light and firm white callus in the dark. Forest Sci. 18:151-154.

Keywords: Pseudotsuga menziesii; tissue culture; vegetative propagation

Document Type: News

Affiliations: Research Associate in the Division of Natural Materials & Systems, and Associate Professor of Forest Biology, The Institute of Paper Chemistry, Appleton, Wisconsin 54911

Publication date: June 1, 1972

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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