Skip to main content

Notes: Callus and Cell Cultures of Douglas-Fir

Buy Article:

$21.50 plus tax (Refund Policy)

Cell suspensions of Douglas-fir doubled in volume weekly, in 300 ft-c of continuous tungsten-fluorescent light. The medium was adapted from Brown and Lawrence and contained 25 mg/liter IAA and 0.5 mg/liter kinetin. On the same medium solidified with agar, callus generally died after 1-2 monthly subcultures, but lived for an additional 1-2 passages when the tungsten light was omitted. The growth hormones were changed to 1-5 mg/liter -naphthoxyacetic acid and 0.0-1 mg/liter N6benzylaminopurine, and the callus was grown in 16 hr of fluorescent light at 25°C, alternating with 8 hr of darkness at 15°C. This has resulted in continuous growth, for over one year, of friable green callus in the light and firm white callus in the dark. Forest Sci. 18:151-154.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics

Keywords: Pseudotsuga menziesii; tissue culture; vegetative propagation

Document Type: News

Affiliations: Research Associate in the Division of Natural Materials & Systems, and Associate Professor of Forest Biology, The Institute of Paper Chemistry, Appleton, Wisconsin 54911

Publication date: 1972-06-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2016 Impact Factor: 1.782 (Rank 17/64 in forestry)

    Average time from submission to first decision: 62.5 days*
    June 1, 2016 to Feb. 28, 2017

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
  • Submit a Paper
  • Membership Information
  • Author Guidelines
  • Podcasts
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more