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Isozyme Specificity During Germination and Early Growth of Knobcone Pine

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Five enzyme classes from 11 developmental stages of germinating embryos were separated by starch gel electrophoresis. Alcohol dehydrogenase isozymes found in embryos of dry seed were most active at the time of radicle emergence; activity decreased thereafter, fading below the level of detection when seed coats were shed. Peroxidase isozymes were absent and esterase isozymes were nearly absent from stratified seed, but both increased steadily in number and stainability from time of radicle emergence through epicotyl elongation. Leucine aminopeptidase and catalase isozymes were detectable in all growth stages. In later stages, esterases and leucine aminopepfidases were found primarily in seedling tops, and many catalase and peroxidase isozymes differed between roots and tops. Both the developmental stage of the plant and the tissue assayed determine the presence of specific isozymes. Forest Sci. 17: 494-498.
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Keywords: Pinus attenuata Lemm; alcohol dehydrogenase; catalase; esterase; leucine aminopeptidase; peroxidase; seed

Document Type: Journal Article

Affiliations: Geneticist, Pacific Southwest Forest and Range Exp. Sta., Forest Service, U.S. Dep. Agr., Berkeley, Calif.

Publication date: 1971-12-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2016 Impact Factor: 1.782 (Rank 17/64 in forestry)

    Average time from submission to first decision: 62.5 days*
    June 1, 2016 to Feb. 28, 2017

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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