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Notes: Tissue Culture Propagation of European Aspen

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Abstract:

Four small trees, 2 to 6 cm tall, were produced from shoots initiated on roots growing from callus tissue of tetraploid European aspen (Populus tremula L.). Firm white callus was initiated in the dark, at 27°C, on a modified Wolter and Skoog defined medium supplemented with amino acids and nucleotides. After 23 bimonthly passages, some inocula were transferred to medium made without auxin, kinetin, or supplements but containing 0.05 mg/liter 6-benzylaminopurine (BAP). Shoots were produced both from callus tissue and roots growing from callus; these shoots rooted and were isolated as independent and normal plants. A method of proliferating and rooting excised shoots is also given. Forest Sci. 17:348-350.

Keywords: Populus tremula; Vegetative reproduction; polyploidy; research techniques

Document Type: News

Affiliations: Research Associate, Institute of Paper Chemistry, Appleton, Wisconsin

Publication date: September 1, 1971

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
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