Notes: Tissue Culture Propagation of European Aspen
Abstract:Four small trees, 2 to 6 cm tall, were produced from shoots initiated on roots growing from callus tissue of tetraploid European aspen (Populus tremula L.). Firm white callus was initiated in the dark, at 27°C, on a modified Wolter and Skoog defined medium supplemented with amino acids and nucleotides. After 23 bimonthly passages, some inocula were transferred to medium made without auxin, kinetin, or supplements but containing 0.05 mg/liter 6-benzylaminopurine (BAP). Shoots were produced both from callus tissue and roots growing from callus; these shoots rooted and were isolated as independent and normal plants. A method of proliferating and rooting excised shoots is also given. Forest Sci. 17:348-350.
Document Type: News
Affiliations: Research Associate, Institute of Paper Chemistry, Appleton, Wisconsin
Publication date: September 1, 1971
More about this publication?
- Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
Forest Science is published bimonthly in February, April, June, August, October, and December.
Also published by SAF:
Journal of Forestry
Other SAF Publications
- Submit a Paper
- Membership Information
- Author Guidelines
- Ingenta Connect is not responsible for the content or availability of external websites