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Protein Synthesis in Sugar Pine Embryos: Isolation and Characterization of a Cell-Free Embryo System

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Abstract:

A cell-free, amino acid-incorporating system has been prepared from embryos of seeds of Pinus lambertiana. Incorporation was dependent on the addition of a synthetic messenger-RNA. Characterization of the incorporation of phenylalanine in the presence of polyuridylic acid showed that optimal incorporation occurred after 45 minutes at pH 8.35 and at 40°. This incorporation was inhibited by ribonuclease, puromycin, and spermine, whereas chloramphenicol, actinomycin D, streptomycin, cycloheximide, and deoxyribonuclease had little effect. Analytical and sucrose density centrifugal analysis of the ribosomal preparations showed the absence of appreciable polyribosomes in embryos from dry seeds. Forest Sci. 16: 212-219.

Keywords: Pinus lambertiana; ribosomes

Document Type: Journal Article

Affiliations: Associate Professor of Biochemistry, Dep of Biochemistry, Virginia Polytechnic Institute, Blacksburg, 24061

Publication date: June 1, 1970

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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