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Notes: A Simple Technique for Investigating Cambial Activity and the Differentiation of Cambial Derivatives

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A sterile culture technique for studying the formation and differentiation of cambial derivatives in isolated stem segments has been successfully developed. One- or two-year-old stem segments (50-70 mm long) are surface-sterilized and their ends aseptically fitted into sterile latex tubing attached to a glass reservoir containing the liquid medium. The stems are fed fresh medium daily by opening and closing clamps above and below the points of their insertion. Stems of Pinus densiflora Sieb. and Zucc. and Larix leptolepis Endl. were grown on a defined medium for 28 days during which time several new xylem elements were formed which differentiated into normal lignified tracheids of the earlywood type. The technique will be useful in studying factors affecting cambial activity and the differentiation of cambial derivatives under controlled nutritional and environmental conditions.

Keywords: Azenic culture; xylem formation

Document Type: News

Affiliations: The Dept. of Forest Botany, Central Univ. of Agriculture, Warsaw, Poland

Publication date: 1969-03-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Average time from submission to first decision: 62.5 days*
    June 1, 2016 to Feb. 28, 2017

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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