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Notes: The Use of Heat-Treated Pollen for Aspen Haploid Production

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Pollen of quaking aspen (Populus tremuloides Michx.) and white poplar (P. alba L.) was treated with infrared heat at 50°C for 0 to 5 minutes, then used to pollinate receptive female flowers of quaking aspen. From the P. tremuloides X tremuloides cross, four haploid diploid mixaploids, but no entirely haploid plants, were recovered from among 2-month-old seedlings. The recovery of spindly, diploid aberrants in both crosses, as well as three glaucous diploids from the P. tremuloides X alba cross, indicates that spontaneous diploidization occurred in maternal haploids. A simple oven is described for weakening pollen.

Document Type: News

Affiliations: Research Associate (and Chief) of the Genetics, Physiology Group, Biology Section, The Institute of Paper Chemistry, Appleton, Wisconsin

Publication date: December 1, 1968

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  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
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