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Notes: The Use of Heat-Treated Pollen for Aspen Haploid Production

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Pollen of quaking aspen (Populus tremuloides Michx.) and white poplar (P. alba L.) was treated with infrared heat at 50°C for 0 to 5 minutes, then used to pollinate receptive female flowers of quaking aspen. From the P. tremuloides X tremuloides cross, four haploid diploid mixaploids, but no entirely haploid plants, were recovered from among 2-month-old seedlings. The recovery of spindly, diploid aberrants in both crosses, as well as three glaucous diploids from the P. tremuloides X alba cross, indicates that spontaneous diploidization occurred in maternal haploids. A simple oven is described for weakening pollen.
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Document Type: News

Affiliations: Research Associate (and Chief) of the Genetics, Physiology Group, Biology Section, The Institute of Paper Chemistry, Appleton, Wisconsin

Publication date: 01 December 1968

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