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Notes: A Simplified Carbowax Embedding Technique for Sectioning Fresh Leaves of Woody Plants

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Cross sections 10-40 thick were rapidly obtained from fresh leaves of woody plants without subjecting tissues to standard fixation-dehydration-infiltration-embedding methods. Water-soluble carbowax (polyethylene glycol 4000) was utilized as a matrix to support leaf tissue for sectioning with a rotary microtome. The relative sensitivity of certain cells to phytotoxic gases was evident in leaf cross sections obtained by this method from Syringa vulgaris L., Pinus strobus L. and Quercus rubra L. that had been exposed to ozone or New York City smog.

Document Type: News

Affiliations: Research Associate, Kitchawan Research Laboratory of the Brooklyn Botanic Garden, 712 Kitchawan Road, Ossining, New York 10562

Publication date: September 1, 1968

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  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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