Notes: A Simplified Carbowax Embedding Technique for Sectioning Fresh Leaves of Woody Plants

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Abstract:

Cross sections 10-40 thick were rapidly obtained from fresh leaves of woody plants without subjecting tissues to standard fixation-dehydration-infiltration-embedding methods. Water-soluble carbowax (polyethylene glycol 4000) was utilized as a matrix to support leaf tissue for sectioning with a rotary microtome. The relative sensitivity of certain cells to phytotoxic gases was evident in leaf cross sections obtained by this method from Syringa vulgaris L., Pinus strobus L. and Quercus rubra L. that had been exposed to ozone or New York City smog.

Document Type: News

Affiliations: Research Associate, Kitchawan Research Laboratory of the Brooklyn Botanic Garden, 712 Kitchawan Road, Ossining, New York 10562

Publication date: September 1, 1968

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