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Colchicine Treatment of Newly Formed Embryos of Quaking Aspen

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A technique is presented for treating newly formed embryos of Populus tremuloides Michx. with colchicine to produce tetraploid plants. Pollinated catkins were immersed in a 0.3 percent solution of colchicine for 6 hours, with treatments starting 6, 12, 18, 24, and 30 hours after pollination. The procedure has the advantage that only the relatively few cells of young embryos are involved in the treatment, thereby greatly increasing the chances of obtaining completely tetraploid individuals.

Document Type: Journal Article

Affiliations: Research Associate, The Inst. of Paper Chemistry, Appleton, Wis.

Publication date: 1965-12-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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