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The Culture of Isolated Triploid Aspen Tissue

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Actively growing aspen tissue can be maintained on a medium containing major elements, trace elements, sucrose, coconut milk, and a-naphthaleneacetic acid. Satisfactory growth was obtained when medium containing a-naphthaleneacetic acid, at concentrations from 0.5 to 8.0 ppm, was supplemented with 100 ppm of casamino acids or malt extract. Cultures may be maintained on agar or in liquid medium. The rate of growth of isolated tissue is increased when the temperature is increased from 25 to 30°C. Aspen tissue may contain areas of red, yellow or green pigmentation. The dominant pigmentation present can be changed by manipulation of the medium and environmental conditions.

Document Type: Journal Article

Affiliations: Research Aide, The Inst. of Paper Chemistry, Appleton, Wis.

Publication date: 1964-03-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 2016 Impact Factor: 1.782 (Rank 17/64 in forestry)

    Average time from submission to first decision: 62.5 days*
    June 1, 2016 to Feb. 28, 2017

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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