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The Culture of Isolated Triploid Aspen Tissue

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Actively growing aspen tissue can be maintained on a medium containing major elements, trace elements, sucrose, coconut milk, and a-naphthaleneacetic acid. Satisfactory growth was obtained when medium containing a-naphthaleneacetic acid, at concentrations from 0.5 to 8.0 ppm, was supplemented with 100 ppm of casamino acids or malt extract. Cultures may be maintained on agar or in liquid medium. The rate of growth of isolated tissue is increased when the temperature is increased from 25 to 30°C. Aspen tissue may contain areas of red, yellow or green pigmentation. The dominant pigmentation present can be changed by manipulation of the medium and environmental conditions.

Document Type: Journal Article

Affiliations: Research Aide, The Inst. of Paper Chemistry, Appleton, Wis.

Publication date: March 1, 1964

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