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Isolation of Fungal Symbionts from Pine Mycorrhizae

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Several successful attempts to isolate the fungal symbiont directly from mycorrhizae of Pinus echinata Mill., P. taeda L., P. elliottii Engelm. var elliottii, and P. palustris Mill, are described. Detergent solution and an ultrasonic bath were employed to clean roots. Surface sterilization with a 1 percent solution of sodium hypochlorite followed. Roots then were rinsed thoroughly with sterile, distilled water. Severed mycorrhizae were plated on nutrient agar media. Recovery in one test was 20-50 percent according to type and pH of medium used. In all, 127 separate pure culture basidiomycetous isolates, including 97 with abundant clamp connections on hyphae, have been isolated from mycorrhizae of 3-month-old to 60-year-old trees growing in sand, sandy loam, and loam soils in the Piedmont and Coastal Plain regions of the Southeast. About 40 different species appear represented. Two have been identified as Clitocybe laccata (Scop. ex Fr.) Quel, and Rhizopogon roseolus. (Corda) Hollos. Also, two isolates of Cenococcum graniforme (Sow.) Fred. and Winge were recovered from slash pine mycorrhizae.

Document Type: Journal Article

Affiliations: Staff of the Southeastern Station at Athens, Ga.

Publication date: 1963-09-01

More about this publication?
  • Forest Science is a peer-reviewed journal publishing fundamental and applied research that explores all aspects of natural and social sciences as they apply to the function and management of the forested ecosystems of the world. Topics include silviculture, forest management, biometrics, economics, entomology & pathology, fire & fuels management, forest ecology, genetics & tree improvement, geospatial technologies, harvesting & utilization, landscape ecology, operations research, forest policy, physiology, recreation, social sciences, soils & hydrology, and wildlife management.
    Forest Science is published bimonthly in February, April, June, August, October, and December.

    2015 Impact Factor: 1.702
    Ranking: 16 of 66 in forestry

    Also published by SAF:
    Journal of Forestry
    Other SAF Publications
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