Several successful attempts to isolate the fungal symbiont directly from mycorrhizae of Pinus echinata Mill., P. taeda L., P. elliottii Engelm. var elliottii, and P. palustris Mill, are described. Detergent solution and an ultrasonic bath were employed to clean roots. Surface sterilization with a 1 percent solution of sodium hypochlorite followed. Roots then were rinsed thoroughly with sterile, distilled water. Severed mycorrhizae were plated on nutrient agar media. Recovery in one test was 20-50 percent according to type and pH of medium used. In all, 127 separate pure culture basidiomycetous isolates, including 97 with abundant clamp connections on hyphae, have been isolated from mycorrhizae of 3-month-old to 60-year-old trees growing in sand, sandy loam, and loam soils in the Piedmont and Coastal Plain regions of the Southeast. About 40 different species appear represented. Two have been identified as Clitocybe laccata (Scop. ex Fr.) Quel, and Rhizopogon roseolus. (Corda) Hollos. Also, two isolates of Cenococcum graniforme (Sow.) Fred. and Winge were recovered from slash pine mycorrhizae.
Document Type: Journal Article
Staff of the Southeastern Station at Athens, Ga.