Free Content Optimization and validation of a chromatographic method for the simultaneous quantification of six bioactive compounds in Rhizoma et Radix Polygoni Cuspidati

Authors: Qian, Guangsheng1; Leung, Sik-Yuen2; Lu, GuangHua2; Leung, Kelvin Sze-Yin3

Source: Journal of Pharmacy and Pharmacology, Volume 60, Number 1, January 2008 , pp. 107-113(7)

Publisher: Pharmaceutical Press

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Abstract:

A reverse-phase HPLC method was developed for simultaneous quantification of six bioactive compounds in Rhizoma et Radix Polygoni Cuspidati. These compounds - polydatin (1), resveratrol (2), rhein (3), emodin (4), chrysophanol (5) and physcion (6) - were analysed from 24 authentic samples of the herb using UV HPLC. Based on the UV absorption characteristics of the six compounds, absorption wavelengths of 306 nm were chosen to quantify compounds 1 and 2, and 290 nm for compounds 3 - 6. A reliable and reproducible quantitative HPLC method for analysing authentic samples of Rhizoma et Radix Polygoni Cuspidati from different cultivation regions was developed. The results showed that the concentration of compound 1 in samples from Sichuan was almost 2 fold higher than that of samples acquired in Guangxi. Furthermore, compounds 3 and 5 were not found in all the samples tested. Thus, instead of using polydatin (1) and emodin (4) as markers for quality assessment, as in conventional practice, these findings show that compounds 2 and 6 are more suited to act as marker compounds for a more specific assessment of the quality of this herb.

Document Type: Research article

DOI: 10.1211/jpp.60.1.0014

Affiliations: 1: Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, P. R. China; West China School of Pharmacy, Sichuan University, No. 17, Section 3, Ren-Mi 2: Research and Development Division, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, P. R. China 3: Department of Chemistry, Hong Kong Baptist University, Kowloon Tong, Kowloon, Hong Kong Special Administrative Region, P. R. China

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