Bilobalide in ginkgo biloba extract is a major substance inducing hepatic CYPs
Authors: Umegaki, Keizo1; Taki, Yuko1; Endoh, Kaori1; Taku, Kyoko1; Tanabe, Hiroki2; Shinozuka, Kazumasa3; Sugiyama, Tomomi4
Source: Journal of Pharmacy and Pharmacology, Volume 59, Number 6, June 2007 , pp. 871-877(7)
Publisher: Pharmaceutical Press
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Abstract:
In a search for substances related to the marked induction of hepatic cytochrome P450 (CYP) by ginkgo biloba extract (GBE), mice were given either GBE (1000 mg kg−1) or fractions of GBE for 5 days. The content and activity of CYPs were induced markedly by a bilobalide-rich fraction, but not by flavonoid-rich fractions. The level of induction by the bilobalide-rich fraction was almost the same as that induced by the unfractionated GBE, suggesting that bilobalide is largely responsible for the CYPs induction. To confirm these findings, mice were given various doses of bilobalide (10.5, 21 and 42 mg kg−1), or GBE (1000 mg kg−1, containing bilobalide at 42 mg kg−1). Treatment with bilobalide induced CYPs markedly and in a dose-dependent manner, and the level of induction was quite similar between bilobalide (42 mg kg−1) and GBE. Treatment with GBE and with bilobalide greatly induced pentoxyresorufin O-dealkylase activity. These findings indicate that bilobalide is the major substance in GBE that induces hepatic CYPs.Document Type: Research article
DOI: 10.1211/jpp.59.6.0014
Affiliations: 1: National Institute of Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, Japan 2: School of Pharmacy, Aichi Gakuin University, 12 Araike, Iwasaki-cho, Nisshin, Aichi, 470-0195, Japan 3: School of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya 663-8179, Japan 4: Faculty of Pharmaceutical Sciences, Hokuriku University, Ho-3, Kanagawa-machi, Kanazawa 920-1181, Japan
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