IngentaConnect The "double-faced" effect of VC-12 on free-radical-induced haemol...

Authors: Li, Guo-Xiang¹; Tang, You-Zhi¹; Liu, Zai-Qun¹

Source: Journal of Pharmacy and Pharmacology, Volume 59, Number 5, May 2007 , pp. 739-743(5)

Abstract:

Vitamin C is a popular antioxidant; however, its water solubility limits its function in the lipid environment. As a result, the antioxidative properties of its lipophilic derivatives have aroused research attention, especially L-ascorbyl-6-laurate (VC-12). We have investigated the effect of a high concentration of VC-12 on 2,2′-azobis(2-amidinopropane hydrochloride) (AAPH)-induced haemolysis of human erythrocytes. The findings indicated that VC-12 was capable of protecting erythrocytes against AAPH-induced haemolysis when its concentration was below 60 μM. With an increase in the concentration of VC-12 and a decrease in the concentration of AAPH, VC-12 promoted haemolysis remarkably, the mechanism of which has been proposed as VC-12-mediated peroxidation. When the concentration of VC-12 was increased to above 150 μM, VC-12 promoted haemolysis by its function as a surfactant, to unbalance the osmotic pressure within and outside erythrocytes. High concentrations of VC-12 may have generated radicals via autooxidation, resulting in eventual haemolysis. Therefore, the double-faced effect of VC-12 on haemolysis of human erythrocytes was due to its concentration. This information may be useful for the safe use of lipophilic vitamin C.

Document Type: Short communication

DOI: 10.1211/jpp.59.5.0016

Affiliations: 1: Department of Organic Chemistry, College of Chemistry, Jilin University, Changchun 130021, China

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The "double-faced" effect of VC-12 on free-radical-induced haemolysis of human erythrocytes: antioxidant and prooxidant