Rubiae Radix suppresses the activation of mast cells through the inhibition of Syk kinase for anti-allergic activity

Authors: Lee, Jun Ho1; Kim, Nam Wook1; Her, Erk1; Kim, Bo Kyung1; Hwang, Keum Hee2; Choi, Dong Kug3; Lim, Beong Ou3; Han, Jeung Whan4; Kim, Young Mi5; Choi, Wahn Soo1

Source: Journal of Pharmacy and Pharmacology, Volume 58, Number 4, April 2006 , pp. 503-512(10)

Publisher: Pharmaceutical Press

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Abstract:

The effect of extracts from various Oriental medicinal herbs on mast-cell-mediated allergic reactions was investigated in this study. Of these extracts, the medicinal herb Rubiae Radix exhibited the most potent activity in the cells, with an IC50 value (concentration necessary to obtain 50% inhibition of the response) of approximately 35 ± 2.1 μg mL−1, and its inhibition of compound-48/80-induced systemic anaphylaxis by 48.6 ± 8.5% at 300 mg kg−1 in mice. It also inhibited the expression of the pro-inflammatory mediator tumour necrosis factor-α (TNF-α). As for its mechanism of action, Rubiae Radix suppressed the activating phosphorylation of Syk, a key enzyme in mast-cell signalling processes, and that of Akt in a dose-dependent manner. It also inhibited the MAP kinase ERK1/2, which is critical for the production of inflammatory cytokines in mast cells, as indicated by the suppression of the activating phosphorylation of ERK1/2. These results suggest that Rubiae Radix suppresses the activation of mast cells through the inhibition of Syk for anti-allergic activity.

Document Type: Research article

DOI: 10.1211/jpp.58.4.0010

Affiliations: 1: College of Medicine and Institute of Biomedical Science and Technology, Konkuk University, Chungju 380-701, Korea 2: Bio-Food and Drug Research Center, Konkuk University, Chungju, 380-701, Korea 3: College of Biomedical & Health Science, Konkuk University, Chungju, 380-701, Korea 4: College of Pharmacy, Sungkyunkwan University, Suwon 440-746, Korea 5: College of Pharmacy, Duksung Women's University, Seoul 132-714, Korea

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