IngentaConnect Inhibitory effects of a fermented ginseng extract, BST204, on the...

Authors: Seo, Jin Young¹; Lee, Jun Ho¹; Kim, Nam Wook¹; Kim, Yong Jun¹; Chang, Sung Ho¹; Ko, Na Young¹; Her, Erk¹; Yoo, Young Hyo²; Kim, Jie Wan²; Lee, Beob Yi³; Lee, Hoi Young⁴; Kim, Young Mi⁵; Choi, Wahn Soo¹

Source: Journal of Pharmacy and Pharmacology, Volume 57, Number 7, July 2005 , pp. 911-918(8)

Publisher: Pharmaceutical Press

Abstract:

In this study, the effects of BST204, a fermented ginseng extract, on the expression of inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production are looked into. Crude ginseng extract was incubated with ginsenoside--glucosidase to prepare BST204. BST204, unlike lipopolysaccharide (LPS) and crude ginseng extract, did not affect the level of iNOS protein and NO production in unstimulated RAW 264.7 cells. However, it suppressed the level of iNOS protein and NO production in LPS-stimulated RAW 264.7 cells but did not manifest the same effect on the iNOS mRNA level. An investigation of the activating phosphorylation of p70 S6 kinase and 4E-BP1, which are important for translation, was conducted to investigate the suppressive mechanism of iNOS protein. LPS increased the phosphorylation of p70 S6 kinase, but not 4E-BP1, in a time-dependent manner, and BST204 inhibited it in a dose-dependent manner. The expression of iNOS protein, however, was partially suppressed by rapamycin, an upstream inhibitor of p70 S6 kinase. Therefore, this paper suggests that the suppression of iNOS protein by BST204 was partially correlated with the inhibition of p70 S6 kinase activation.

Document Type: Research article

DOI: 10.1211/0022357056497

Affiliations: 1: Department of Immunology, College of Medicine and Institute of Biomedical Science and Technology, Konkuk University, Chungju, 380-701, Korea 2: Biosapogen, Gyeonggi-Do 462-120, Korea 3: Department of Anatomy, College of Medicine, Konkuk University, Chungju 380-701, Korea 4: College of Medicine, Konyang University, Nonsan 320-711, Korea 5: College of Pharmacy, Duksung Women's University, Seoul 132–714, Korea

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Inhibitory effects of a fermented ginseng extract, BST204, on the expression of inducible nitric oxide synthase and nitric oxide production in lipopolysaccharide-activated murine macrophages