Effects of phenylethanoid glycosides from Digitalis purpurea L. on the expression of inducible nitric oxide synthase
Authors: Oh, Jae Wook1; Lee, Jeong Yong2; Han, Song Hee2; Moon, Young Hee2; Kim, Yoon Gyoon2; Woo, Eun-Rhan2; Kang, Keon Wook3
Source: Journal of Pharmacy and Pharmacology, Volume 57, Number 7, July 2005 , pp. 903-910(8)
Publisher: Pharmaceutical Press
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Abstract:
We have isolated four different phenylethanoid glycosides (purpureaside A, desrhamnosyl acteoside, calceolarioside B and plantainoside D) from the leaves of Digitalis purpurea (foxglove). The effects of these glycosides on activator protein-1 (AP-1)-mediated inducible nitric oxide synthase (iNOS) gene expression in the Raw264.7 macrophage cell line have been studied. Of these four glycosides, purpureaside A potently inhibited iNOS induction by lipopolysaccharide (LPS). Increase in iNOS mRNA by LPS was completely suppressed by purpureaside A. Purpureaside A did not significantly affect LPS-inducible nuclear factor-
B (NF-B) activation or the nuclear translocation of p65. Moreover, a reporter gene assay using AP-1 specific luciferase reporter revealed that the enhanced activity of AP-1 by LPS was completely abolished in cells treated with purpureaside A. These results demonstrated that purpureaside A inhibited LPS-inducible iNOS expression in macrophages through the suppression of AP-1, but not of NF-B.
Document Type: Research article
DOI: 10.1211/0022357056451
Affiliations: 1: Research Center for Resistant Cells and Department of Anatomy, College of Medicine, Chosun University, Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea 2: College of Pharmacy, Chosun University, Seosuk-dong, Dong-gu, Gwangju 501-759, South Korea 3: Department of Pharmacology, College of Medicine, Dangook University, Cheonan 330-714, South Korea
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