Abstract:

We investigated the effect of diethylhexylphthalate (DEHP) from Aloe vera Linne on the apoptosis of human leukaemic cell lines K562, HL60 and U937 to examine its pharmacological activity.

At a level of 10 g mL^-1 DEHP a significant anti-leukaemic effect was observed for all three cell lines, as measured by clonogenic assay. After treatment with 10 g mL^-1 DEHP for 4 h, agarose gel electrophoresis and flow cytometric analysis confirmed the occurrence of apoptosis.

These results indicate that DEHP isolated from Aloe vera Linne has a potent anti-leukaemic effect, and thus represents a new type of pharmacological activity with respect to human leukaemic cells.

Document Type: Research article

DOI: 10.1211/0022357001774778

Affiliations: 1: Animal Resource Research Center, Konkuk University, 93-1, Mojin-dong, Kwangjin-ku, Seoul, 143-701, Korea, chhan@kkucc.konkuk.ac.kr 2: Laboratory of Pharmacology, Ewha Women’s University Mokdong Hospital, 911-1 Mok-6-dong, Yangchon-ku, Seoul, 158-710, Korea