Effect of Aegle marmelos on Biotransformation Enzyme Systems and Protection Against Free-radical-mediated Damage in Mice
Authors: Singh R.P.1; Banerjee S.1; Ramesha Rao A.1
Source: Journal of Pharmacy and Pharmacology, Volume 52, Number 8, 1 August 2000 , pp. 991-1000(10)
Publisher: Pharmaceutical Press
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Abstract:
The effect of hydroalcoholic (80% ethanol, 20% water) extract of leaves of Aegle marmelos was examined on carcinogen-metabolizing phase-I and phase-II enzymes, antioxidant enzymes, glutathione content, lactate dehydrogenase and lipid peroxidation, using two doses of dried extract (50 and 100 mg kg-1 daily for 14 days), in the liver of mice. The modulatory effect of the extract was also examined on extrahepatic organs (lung, kidney and fore-stomach) for effects on the activity of glutathione S-transferase, DT-diaphorase, superoxide dismutase and catalase.Extract treatment significantly increased the basal levels of acid-soluble sulphydryl (–SH) content, cytochrome P450, NADPH-cytochrome P450 reductase, cytochrome b5, NADH-cytochrome b5 reductase, glutathione S-transferase, DT-diaphorase, superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase in the liver. Aegle acted as a bifunctional inducer since it induced both phase-I and phase-II enzyme systems. Both doses significantly decreased the activity of lactate dehydrogenase and formation of malondialdehyde in liver, suggesting a role in cytoprotection as well as protection against pro-oxidant-induced membrane damage. Butylated hydroxyanisole (positive control) induced almost all the antioxidative parameters measured in this study. The extract was effective in inducing glutathione S-transferase, DT-diaphorase, superoxide dismutase and catalase in lung, glutathione S-transferase, DT-diaphorase and superoxide dismutase in fore-stomach, and DT-diaphorase and superoxide dismutase in lung.These significant changes in the levels of drug-metabolizing enzymes and antioxidative profiles are strongly indicative of the chemopreventive potential of this plant, especially against chemical carcinogenesis.Document Type: Research article
DOI: 10.1211/0022357001774714
Affiliations: 1: Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India, aramesharao@yahoo.com
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