EST-SSRS developed for other Picea species amplify in Picea omorika and reveal high genetic variation in two natural populations

$23.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Picea omorika (Panč.) Purk. is a relict and an endemic species found exclusively in the Balkan, within an area of ca. 10 000 km2. Marker-based genetic diversity data in this conifer are very limited and partially contradictory. Therefore, twelve nuclear markers (ten EST-SSRs and two genomic SSRs) were tested for cross-species amplification in P. omorika. Five EST-SSRs amplified successfully and a very high number of alleles per locus was found in 50 trees originating from two natural populations (7 to 18 alleles per locus), with a total of 61 alleles. Furthermore, a high number of private alleles were detected – 13 and 14 per population, respectively. Rare alleles, i.e., alleles whose frequency was lower than 0.05, were not observed. Markers were selectively neutral, no linkage disequilibrium was detected and the genotype frequencies fitted Hardy–Weinberg proportions. Expected heterozygosity per locus ranged from 0.64 to 0.91 in both populations, with an overall mean of 0.83. Considering the small remnant population sizes of P. omorika, these values are unexpectedly high and comparable to values in P. glauca (Moench) Voss., P. sitchensis (Bong.) Carr. and P. mariana (Mill.) B.S.P. detected with an identical set of markers in samples of similar sizes.

Keywords: ENDEMIC SPECIES; EST-SSRS; GENETIC DIVERSITY; GENOMIC SSRS; MICROSATELLITES; PICEA OMORIKA

Document Type: Short Communication

Publication date: September 1, 2009

More about this publication?
  • The Belgian Journal of Botany (now known as Plant Ecology and Evolution) is an international journal open to all fields of plant sciences. Please note, however, that papers restricted to purely nomenclatural matters or to floristical data of only local interest will not be accepted. The Journal appears in one volume of two issues per year. It publishes reviews, original research papers, short notes, letters to the editor, and book reviews. Click here for current issues of this journal
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Membership Information
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more