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Direct regeneration of rattan seedlings from apical meristem and axillary bud explants

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Abstract:

A study was conducted to develop a protocol for mass-propagation of an economically important rattan species [Laccosperma secundiflorun (P. Beauv.) Kuntze] using leaves, axillary bud, and apical meristem explants collected from natural stands. The explants were incubated on basal MS (Murashige & Skoog 1962) medium containing vitamins (MSV), supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP) and α-naphthaleneacetic acid (NAA). Best results (100% vigorous explants) from initiation phase were obtained after four weeks with axillary bud and apical meristem explants cultured in darkness at 28 ± 2°C on MSV supplemented with 30 g L−1 sucrose and 0.65 mg L−1 BAP. MSV containing 1 mg L−1 BAP and 1 mg L−1 NAA allowed highest rates of explants forming shoots (> 85%), with a mean of 3 shoots per explant after eight weeks. A high rate (> 95%) of shoots generated well-developed roots after two weeks on MSV containing 0.5 mg L−1 3-indoleacetic acid (IAA).

Keywords: CALAMOIDEAE; IN VITRO CULTURE; LACCOSPERMA SECUNDIFLORUN; RATTAN PALM; SHOOT REGENERATION

Document Type: Regular Paper

Publication date: 2009-09-01

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  • The Belgian Journal of Botany (now known as Plant Ecology and Evolution) is an international journal open to all fields of plant sciences. Please note, however, that papers restricted to purely nomenclatural matters or to floristical data of only local interest will not be accepted. The Journal appears in one volume of two issues per year. It publishes reviews, original research papers, short notes, letters to the editor, and book reviews. Click here for current issues of this journal
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