Isolation and characterization of microsatellite loci in primrose (Primula vulgaris)

Authors: Van Geert, Anja; Van Rossum, Fabienne; Stiers, Iris; Sierens, Tim; Barker, Jacqueline H.A.; Triest, Ludwig

Source: Belgian Journal of Botany, Volume 139, Number 2, December 2006 , pp. 261-264(4)

Publisher: Royal Botanical Society of Belgium

Buy & download fulltext article:

Price: $23.00 plus tax (Refund Policy)

Abstract:

Microsatellite loci were developed for Primula vulgaris, a distylous, insect-pollinated, long-lived perennial herb. Primula vulgaris is rare and declining in Belgium. Eighteen microsatellite primer pairs were tested on 267 P. vulgaris individuals and on 246 individuals of its common congener Primula elatior to test for cross-species amplification. Only three polymorphic microsatellite loci amplified successfully in P. vulgaris and only two in P. elatior. Between 4 and 11 alleles were found per locus in P. vulgaris and 8-11 alleles per locus in P. elatior. Expected heterozygosity ranged from 0.496-0.620 and from 0.830-0.880 for P. vulgaris and P. elatior, respectively.

Keywords: PRIMULA ELATIOR; PRIMULA VULGARIS; CONSERVATION GENETICS; GENE FLOW; HABITAT FRAGMENTATION; SSR

Document Type: Short communication

Publication date: 2006-12-01

More about this publication?

The Belgian Journal of Botany (now known as Plant Ecology and Evolution) is an international journal open to all fields of plant sciences. Please note, however, that papers restricted to purely nomenclatural matters or to floristical data of only local interest will not be accepted. The Journal appears in one volume of two issues per year. It publishes reviews, original research papers, short notes, letters to the editor, and book reviews. Click here for current issues of this journal
Editorial Board
Information for Authors
Submit a Paper
Membership Information
ingentaconnect is not responsible for the content or availability of external websites