@article {Bhatt:February 2007:0021-9665:91,
	author = "Bhatt, Jignesh",
	author = "Shah, Bhavin",
	author = "Kambli, Sandeep",
	author = "Subbaiah, Gunta",
	author = "Singh, Sadhana",
	author = "Ameta, Suresh",
	title = "Rapid and Sensitive Method for the Determination of Sibutramine Active Metabolites in Human Plasma by Reversed-Phase Liquid ChromatographyTandem Mass Spectroscopy",
	journal = "Journal of Chromatographic Science",
	volume = "45",
	year = "February 2007",
	abstract = "A new, rapid, and sensitive liquid chromatography-tandem mass spectrometry method is developed and validated to quantitate the sibutramine active metabolites mono desmethyl sibutramine (M<SUB>1</SUB>) and di-desmethyl sibutramine (M<SUB>2</SUB>) using imipramine as the internal standard in human plasma samples for routine bioequivalence studies. The method involves rapid solid-phase extraction from plasma, eliminating the drying and reconstitution steps. The analytes are chromatographed on a C<SUB>8</SUB> reversed-phase chromatographic column and analyzed by mass spectrometry in the multiple reaction monitoring mode, which enables a quantitation limit at the sub-nanogram level. The method has a chromatographic run time of 2.8 min. The proposed method is validated with a linear range of 0.1-8.0 and 0.2-16.0 ng/mL for M<SUB>1</SUB> and M<SUB>2</SUB>, respectively, with a correlation coefficient of regression &#8805; 0.9990. The method is sensitive and reproducible, having intra- and inter-assay precision at the lower limit of quantitation (0.1 ng/mL for M<SUB>1</SUB> and 0.2 ng/mL for M<SUB>2</SUB>) &#60; 10.0%. The overall recovery for M<SUB>1</SUB> and M<SUB>2</SUB> is 93.5% and 77.9%, respectively. The method has been applied to a bioequivalence clinical study with great success.",
	pages = "91-96(6)",
	url = "http://www.ingentaconnect.com/content/pres/jcs/2007/00000045/00000002/art00006"
}