IngentaConnect Rapid and Sensitive Method for the Determination of Sibutramine A...

Authors: Bhatt, Jignesh¹; Shah, Bhavin²; Kambli, Sandeep²; Subbaiah, Gunta²; Singh, Sadhana³; Ameta, Suresh⁴

Source: Journal of Chromatographic Science, Volume 45, Number 2, February 2007 , pp. 91-96(6)

Abstract:

A new, rapid, and sensitive liquid chromatography-tandem mass spectrometry method is developed and validated to quantitate the sibutramine active metabolites mono desmethyl sibutramine (M₁) and di-desmethyl sibutramine (M₂) using imipramine as the internal standard in human plasma samples for routine bioequivalence studies. The method involves rapid solid-phase extraction from plasma, eliminating the drying and reconstitution steps. The analytes are chromatographed on a C₈ reversed-phase chromatographic column and analyzed by mass spectrometry in the multiple reaction monitoring mode, which enables a quantitation limit at the sub-nanogram level. The method has a chromatographic run time of 2.8 min. The proposed method is validated with a linear range of 0.1-8.0 and 0.2-16.0 ng/mL for M₁ and M₂, respectively, with a correlation coefficient of regression ≥ 0.9990. The method is sensitive and reproducible, having intra- and inter-assay precision at the lower limit of quantitation (0.1 ng/mL for M₁ and 0.2 ng/mL for M₂) < 10.0%. The overall recovery for M₁ and M₂ is 93.5% and 77.9%, respectively. The method has been applied to a bioequivalence clinical study with great success.

Document Type: Research article

Affiliations: 1: Torrent Research Centre, Gandhinagar-382428, Gujarat State, India; and Department of Chemistry, B.N.P.G. College, Mohanlal Sukhadia University, Udaipur, Rajasthan State, India 2: Torrent Research Centre, Gandhinagar-382428, Gujarat State, India 3: Department of Chemistry, B.N.P.G. College, Mohanlal Sukhadia University, Udaipur, Rajasthan State, India 4: Department of Chemistry, College of science, Mohanlal Sukhadia University, Udaipur, Rajasthan State, India

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Rapid and Sensitive Method for the Determination of Sibutramine Active Metabolites in Human Plasma by Reversed-Phase Liquid Chromatography-Tandem Mass Spectroscopy