Determination of Aldehydes in Fish by High-Performance Liquid Chromatography

Authors: da Cunha Veloso M.C.¹; da Silva V.M.²; Santos G.V.³; de Andrade J.B.⁴

Source: Journal of Chromatographic Science, Volume 39, Number 5, May 2001 , pp. 173-176(4)

Publisher: Preston Publications

Abstract:

A new analytical method to determine trace volatile aldehydes isolated from the headspace of fish meat at room temperature by high-performance liquid chromatography (HPLC) in the form of 2,4-dinitrophenylhydrazone (DNPHo) derivatives has been developed. Aliquots (50 g) of the fish purée were introduced into a 500-mL glass recipient and were purged with N₂ for 40 min through two SEP-PAK C18 cartridges (connected in series) coated with an acid solution of 2,4-dinitrophenylhydrazine. The cartridges were then eluted with acetonitrile (2 mL) and the 2,4-DNPHo formed was quantitated by HPLC–UV analysis using a Zorbax C18 column. The isolated compounds from the dynamic headspace sampling of four kinds of fish species were saturated aldehydes, formaldehyde, acetaldehyde, propanal, butanal, pentanal, and hexanal. Under optimized conditions the detection limits of the HPLC method were in the range of 0.75 nmol/g (formaldehyde) to 2.19 nmol/g (hexanal). The calibration curves were linear in the concentration range from 1.3 nmol/mL to 12.5 nmol/mL. Propanal and acetaldehyde were the major carbonyl compounds identified (ranging from 3.9 nmol/g and 10 nmol/g). This study has revealed the widespread occurrence of formaldehyde, acetaldehyde, propanal, butanal, pentanal, and hexanal in fish meat.

Language: English

Document Type:

Affiliations: 1: Instituto de Química, Universidade Federal da Bahia – UFBA, 40170-290, Salvador, Bahia, Brazil, Centro Federal de Educaço Tecnológica da Bahia – CEFET o Tecnológica da Bahia – CEFET "> 2: Instituto de Química, Universidade Federal da Bahia – UFBA, 40170-290, Salvador, Bahia, Brazil 3: Instituto de Química, Universidade Federal da Bahia – UFBA, 40170-290, Salvador, Bahia, Brazil, Instituto de Biologia – UFBA 4: Instituto de Química, Universidade Federal da Bahia – UFBA, 40170-290, Salvador, Bahia, Brazil, Email: jailsong@ufba.br

Publication date: 2001-05-01

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