An Enzyme-Linked Immunosorbent Assay (ELISA) for Methylphenidate (Ritalin®) in Urine
Authors: Lewis M.G.; Lewis J.G.; Elder P.A.; Moore G.A.
Source: Journal of Analytical Toxicology, Volume 27, Number 6, September 2003 , pp. 342-345(4)
Publisher: Preston Publications
Abstract:
A direct enyzme-linked immunosorbent assay (ELISA) for urinary immunoreactive methylphenidate (Ritalin), in which a standard 96-well microtiter plate is used, is described. For this ELISA, a methylphenidate-thyroglobulin conjugate is immobilized to the microtiter plate and competes with methylphenidate in the standard or urine sample for antibody-binding sites. After washing, the sheep methylphenidate antibody bound to immobilized methylphenidate is detected with peroxidase-labelled goat antisheep IgG. Following a further wash, tetramethylbenzidine is added, color is developed, and the plate is read at 450 nm on an ELISA plate reader. This method is unaffected by drugs of abuse and is suitable for routine use in the toxicology laboratory.Document Type: Research article
Affiliations: 1: Canterbury Health Laboratories, Christchurch, New Zealand
Publication date: 2003-09-01
The Journal of Analytical Toxicology (JAT), established in 1977 and published 9 times a year, is the international source covering a broad range of clinical, forensic, and industrial laboratory topics regarding the isolation, identification, and quantitation of potentially toxic substances.
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