IngentaConnect Determination of Methamphetamine and Its Metabolites Incorporated...

Authors: Miki A.¹; Katagi M.¹; Tsuchihashi H.¹

Source: Journal of Analytical Toxicology, Volume 27, Number 2, March 2003 , pp. 95-102(8)

Abstract:

An automated column-switching liquid chromatographic&Eth; electrospray ionization-mass spectrometric (LC–ESI–MS) method has been established for the determination of methamphetamine (MA) and its metabolites, amphetamine (AP), and p-hydroxymethamphetamine (p–OH–MA) in hair. The combination of an N–vinylacetamide-containing hydrophilic polymer online extraction column, an SCX semi-micro LC column, and an electrospray ionization interface provided the successful concentration, separations, and highly sensitive MS determinations of these analytes in a hair extract without tedious sample pretreatments. The limits of detection of these analytes were 0.02 ng/mg and 0.1–0.2 ng/mg in the selected-ion monitoring (SIM) and full-scan modes, respectively, when using a 100-

L hair extract sample that corresponds to a 2.5-mg sample of hair. The calibration curves using dibenzylamine as an internal standard were linear up to 30 ng/mg hair equivalents for all these analytes in the SIM mode. p–OH–MA, the detection of which in MA users' hair had not been previously reported, was detectable in all 22 hair specimens/sections from which 1 ng or more of MA was detected per milligram hair. The amount of p–OH–MA detected per milligram of hair is presented with those of MA and AP among the MA users population. The detection of AP and p–OH–MA, in addition to the parent drug MA with reasonable ratios, was found to be a useful indicator for distinguishing internal MA incorporation from external contamination.

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Determination of Methamphetamine and Its Metabolites Incorporated in Hair by Column-Switching Liquid Chromatography-Mass Spectrometry