IngentaConnect TECHNICAL NOTE: Determination of Cobalt in Urine by FI-ICP-AES wi...

Authors: Farias G.M.¹; Wuilloud R.G.¹; Moyano S.¹; Gásquez J.A.¹; Olsina R.A.^{1, 2}; Martinez L.D.^{1, 2}

Source: Journal of Analytical Toxicology, Volume 26, Number 6, September 2002 , pp. 360-364(5)

Abstract:

A method for the preconcentration and determination of cobalt in human urine samples was developed. The online preconcentration and determination were attained using inductively coupled plasma atomic emission spectromety (ICP-AES) coupled to a flow injection (FI) method. Cobalt was retained on an Amberlite XAD-7 resin as cobalt-2-(5-bromo-2-pyridylazo)-5-diethylaminophenol complex at pH 9.5. Cobalt was removed from the microcolumn with perchloric acid. A sensitivity enhancement factor of 90 was obtained with respect to cobalt determination by ICP-AES without preconcentration. The value of detection limit for the preconcentration method proposed was 25 ng/L. The precision for 10 replicate determinations at the 5 mg/L (mean ± SD, 5.1 ± 0.14) Co level was 2.7% relative standard deviation, calculated from the peak heights obtained. The calibration graph preconcentration method for cobalt was linear with a correlation coefficient of 0.9994 from approximately 0.25 mg/L up to at least 100 mg/L. The method was successfully applied to the determination of cobalt in human urine samples.

Language: English

Document Type:

Affiliations: 1: Department of Analytical Chemistry, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, P.O. Box 375, 5700 San Luis, Argentina 2: Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Rivadavia 1917, CP C1033 AAJ, Cuidad de Buenos Aires, Argentina

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TECHNICAL NOTE: Determination of Cobalt in Urine by FI-ICP-AES with Online Preconcentration