IngentaConnect Application of EMIT(R) d.a.u.(TM) for the Semiquantitative Screen...

Authors: Miki A.¹; Katagi M.¹; Tsuchihashi H.¹

Source: Journal of Analytical Toxicology, Volume 26, Number 5, July/August 2002 , pp. 274-279(6)

Abstract:

By the use of an enzyme multiplied immunoassay technique (EMIT), a convenient and fairly sensitive semiquantitative screening method was established for methamphetamine (MA) incorporated in hair. MA in a 10-mg portion of hair was extracted into 5M HCl/methanol (1:20, v/v), and the extract reconstituted in 100 µL water was assayed with double-concentrated Emit d.a.u. Amphetamine Class assay reagents. The optimal cutoff concentration of MA in hair was found by receiver operating characteristic analysis to be 1.0 ng/mg, and the detection limit was calculated to be 0.5 ng/mg. The semiquantitative screening was possible over the concentration range from 1.0 to 200 ng/mg, and the results are in good agreement with those by gas chromatographic?mass spectrometric (GC?MS) determination. Although all positive results must be confirmed by either GC?MS or a specific alternative methodology, this method not only provided reliable screening suitable for drug enforcement purposes, but it also allowed sectional profile analysis of MA in hair while requiring only a 10-mg hair specimen.

Language: English

Document Type:

Affiliations: 1: Forensic Science Laboratory, Osaka Prefectural Police Headquarters, 1-3-18 Hommachi, Chuo-ward, Osaka 541-0053, Japan

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Application of EMIT(R) d.a.u.(TM) for the Semiquantitative Screening of Methamphetamine Incorporated in Hair