Authors: Miki, A.; Katagi, M.; Tsuchihashi, H.

Source: Journal of Analytical Toxicology, Volume 26, Number 5, 1 July 2002 , pp. 274-279(6)

Publisher: Preston Publications

Abstract:

By the use of an enzyme multiplied immunoassay technique (EMIT), a convenient and fairly sensitive semiquantitative screening method was established for methamphetamine (MA) incorporated in hair. MA in a 10-mg portion of hair was extracted into 5M HCl/methanol (1:20, v/v), and the extract reconstituted in 100 µL water was assayed with double-concentrated Emit d.a.u. Amphetamine Class assay reagents. The optimal cutoff concentration of MA in hair was found by receiver operating characteristic analysis to be 1.0 ng/mg, and the detection limit was calculated to be 0.5 ng/mg. The semiquantitative screening was possible over the concentration range from 1.0 to 200 ng/mg, and the results are in good agreement with those by gas chromatographic-mass spectrometric (GC-MS) determination. Although all positive results must be confirmed by either GC-MS or a specific alternative methodology, this method not only provided reliable screening suitable for drug enforcement purposes, but it also allowed sectional profile analysis of MA in hair while requiring only a 10-mg hair specimen.

Document Type: Research article

Affiliations: 1: Forensic Science Laboratory, Osaka Prefectural Police Headquarters, 1-3-18 Hommachi, Chuo-ward, Osaka 541-0053, Japan

Publication date: 2002-07-01

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• The Journal of Analytical Toxicology (JAT), established in 1977 and published 9 times a year, is the international source covering a broad range of clinical, forensic, and industrial laboratory topics regarding the isolation, identification, and quantitation of potentially toxic substances.

  With an emphasis on practical application, JAT articles provide improved and novel techniques for use in clinical, forensic, workplace, sports testing (doping), and other toxicology laboratories. Articles describe newly developed methods in immunoassay testing, gas chromatography, liquid chromatography, mass spectrometry, atomic absorption spectrometry, solid and liquid phase extraction techniques, and other analytical approaches. Worldwide readership includes toxicologists, pathologists, chemists, clinicians, researchers, and educators working in medical examiner and law enforcement laboratories, hospitals, university and independent analytical laboratories, as well as the drug manufacturing industry.

  Each year in October, we publish a special issue from the Society of Forensic Toxicologists.

  JAT, as determined by ISI Citation Index, is one of the two most referenced international journals in forensic science.

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