Cobas Mira™ S Endpoint Enzymatic Assay for Plasma Formate
Authors: Blomme B.1; Lheureux P.2; Gerlo E.1; Maes V.1
Source: Journal of Analytical Toxicology, Volume 25, Number 2, March 2001 , pp. 77-80(4)
Publisher: Preston Publications
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Abstract:
In methanol intoxication, increased levels of the metabolite formate are associated with metabolic acidosis and an increased risk for ocular and neurological dysfunction. A simple method for plasma formate measurement by adaptation of a manual enzymatic assay to a Cobas Mira S analyzer is presented. Six microliters of sample is incubated for 5 min with buffer containing nicotinamide-adenine dinucleotide. Fifteen microliters of a suspension of formate dehydrogenase is then added. Absorbance at 340 nm is measured every 25 s. The NADH produced when formate is oxidized is stoichiometric to the amount of formate. The method is sensitive, reproducible, and specific and has a broad measurement range. The frozen reagents are stable for at least six months, so the described method can be applied to irregular and semi-urgent requests. A recent case is reported.
Language: English
Document Type:
Affiliations: 1: Department of Clinical Chemistry–Toxicology, Academic Hospital Free University Brussels, VUB, Laarbeeklaan 101, B-1090 Brussels, Belgium 2: Emergency Department, Erasme Hospital, ULB, Route de Lennik 808, B-1070 Brussels, Belgium
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