Affinity chromatography system for parallel purification of recombinant protein samples
Authors: Gottstein, C.1; Forde, R.2
Source: Protein Engineering, Volume 15, Number 10, October 2002 , pp. 775-777(3)
Publisher: Oxford University Press
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Abstract:
In protein engineering, the tasks of generating and testing a large number of variants of a molecule and of optimizing expression conditions for one distinct molecule create the need for purification methods that can handle a large number of samples simultaneously. We describe the development and some application results of a simple affinity chromatography system that can be used for the parallel purification of 24 protein samples, yielding sufficient quantities for biochemical and functional analysis. Advantages of this system over existing systems are as follows. Compared with commercially available complete chromatography systems, the costs of this system are minimal. In comparison with vacuum systems with various outlets, and with batch purification systems where centrifugation is necessary, this system allows gentler processing of the samples. This could be important for proteins that are easily damaged.Keywords: affinity chromatography; chromatography system; expression conditions optimization; parallel protein purification; recombinant proteins
Document Type: Research article
Affiliations: 1: Department of Internal Medicine I, Laboratory for Tumortherapy, University of Cologne, 50924 Cologne and 2: Aqua Research, 50974 Cologne, Germany
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