Protein Engineering

Silent mutations in secondary Shine–Dalgarno sequences in the cDNA of human serum amyloid A4 promotes expression of recombinant protein in Escherichia coli
pp. 949-952(4)
Authors: Hrzenjak, Andelko; Artl, Andreas; Knipping, Gabriele; Kostner, Gerhard; Sattler, Wolfgang

Exploring the unique pharmacology of a novel opioid receptor, ZFOR1, using molecular modeling and the `message–address' concept
pp. 953-960(8)
Authors: McFadyen, Iain J.; Metzger, Thomas G.; Paterlini, M.Germana

High thermal stability of 3-isopropylmalate dehydrogenase from Thermus thermophilus resulting from low C_p of unfolding
pp. 961-966(6)
Authors: Motono, Chie; Oshima, Tairo

Energetics of three-state unfolding of a protein: canine milk lysozyme
pp. 967-974(8)
Authors: Koshiba, Takumi; Kobashigawa, Yoshihiro; Demura, Makoto

Heat labile ribonuclease HI from a psychrotrophic bacterium: gene cloning, characterization and site-directed mutagenesis
pp. 975-982(8)
Authors: Ohtani, Naoto; Haruki, Mitsuru; Morikawa, Masaaki

Kinetic characterization of ribonuclease S mutants containing photoisomerizable phenylazophenylalanine residues
pp. 983-991(9)
Authors: James, D.Andrew; Burns, Darcy C.

Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency
pp. 993-1000(8)
Authors: Kapust, Rachel B.; Tözsér, József; Fox, Jeffrey D.; Anderson, D.Eric; Cherry, Scott; Copeland, Terry D.

Metal binding affinity and structural properties of an isolated EF-loop in a scaffold protein
pp. 1001-1013(13)
Authors: Ye, Yiming; Lee, Hsiau-Wei; Yang, Wei; Shealy, Sarah J.; Wilkins, Anna L.; Liu, Zhi-ren; Torshin, Ivan; Harrison, Robert; Wohlhueter, Robert

Interactions between HMG boxes
pp. 1015-1023(9)
Authors: Taudte, Susann; Xin, Hong; Bell, Anthony J.

Multimerization of a chimeric anti-CD20 single-chain Fv-Fc fusion protein is mediated through variable domain exchange
pp. 1025-1033(9)
Authors: Wu, Anna M.; Tan, Giselle J.; Sherman, Mark A.; Clarke, Patrick; Olafsen, Tove; Forman, Stephen J.

Improved binding of a bivalent single-chain immunotoxin results in increased efficacy for in vivo T-cell depletion
pp. 1035-1041(7)
Authors: Thompson, Jerry; Stavrou, Scott; Weetall, Marla; Hexham, J.Mark; Digan, Mary Ellen; Wang, Zhuri; Woo, Jung Hee; Yu, Yongjun; Mathias, Askale; Liu, Yuan Yi; Ma, Shenglin; Gordienko, Irina; Lake, Philip

Selection of catalytically active biotin ligase and trypsin mutants by phage display
pp. 1043-1052(10)
Authors: Heinis, Christian; Huber, Adrian; Demartis, Salvatore; Bertschinger, Julian; Melkko, Samu; Lozzi, Luisa; Neri, Paolo

Optimal classification of protein sequences and selection of representative sets from multiple alignments: application to homologous families and lessons for structural genomics
pp. 1053-1053(1)