@article {Nakanomyo:15 December 2002:0032-0781:1484,
	author = "Nakanomyo I.",
	author = "Kost B.",
	author = "Chua N-H.",
	author = "Fukuda H.",
	title = "Preferential and Asymmetrical Accumulation of a Rac Small GTPase mRNA in Differentiating Xylem Cells of Zinnia elegans",
	journal = "Plant and Cell Physiology",
	volume = "43",
	year = "15 December 2002",
	abstract = "<P>Rac-type small GTPases are known to function in some cellular processes in plants. To further understand the involvement of Rac type GTPases in plant development, we isolated from cultured <I>Zinnia</I> cells a gene (<I>ZeRAC2</I>) encoding a new Rac-type small GTPase. <I>ZeRAC2</I> mRNA accumulates preferentially in xylogenic culture and transiently at the time when visible tracheary elements appear. Experiments with ZeRAC2 recombinant proteins demonstrated that ZeRAC2 binds to and hydrolyzes GTP. A GFP&#150;ZeRAC2 fusion protein was localized to the plasma membrane. Together with the fact that ZeRAC2 possesses a putative geranylgeranylation site at the C-terminus, this suggests that ZeRAC2 acts on the plasma membrane. In situ hybridization indicated that <I>ZeRAC2</I> mRNA accumulates preferentially in xylem parenchyma and tracheary element precursor cells, and surprisingly the accumulation is restricted to the site facing developing tracheary elements.</P>",
	pages = "1484-1492(9)",
	url = "http://www.ingentaconnect.com/content/oup/pcp/2002/00000043/00000012/art01484"
}