Authors: Yoshizaki, Takayuki¹; Ohtani, Katsuki¹; Motomura, Wataru¹; Jang, Seong-Jae¹; Mori, Ken-ichiro¹; Kitamoto, Noritoshi²; Yoshida, Itsuro¹; Suzuki, Yasuhiko³; Wakamiya, Nobutaka¹

Source: The Journal of Biochemistry, Volume 151, Number 1, January 2012 , pp. 57-64(8)

Publisher: Oxford University Press

Abstract:

Mannan-binding lectin (MBL) was first discovered as a collectin in animal blood, and was shown to have such unique characteristics as a collage-like domain and a carbohydrate recognition domain. We recently identified human collectin kidney 1 (CL-K1, COLEC11) from a human kidney cDNA library. To quantitate the CL-K1 concentration in blood, we developed several polyclonal and monoclonal antibodies using recombinant human CL-K1 in CHO cells and the CL-K1 fragment in Escherichia coli. Using these antibodies, we established a sandwich enzyme-linked immunosorbent assay (ELISA) system. The concentration of CL-K1 in human plasma was 0.34 0.13 g/ml and that in MBL was 1.72 1.51 g/ml. Concentrations of MBL are often low due to its single nucleotide polymorphisms (SNPs) which seem to be related to an opsonic defect. However, no low concentrations of CL-K1 were observed on testing over two hundred blood samples. We also found that the blood concentration of CL-K1 was not dependent on gender or age and did not correlate completely with that of MBL. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of CL-K1 in humans.

Keywords: complement; blood coagulation; lectin; carbohydrate; genetic

Document Type: Regular paper

DOI: http://dx.doi.org/10.1093/jb/mvr114

Affiliations: 1: Department of Microbiology and Immunochemistry, Asahikawa Medical University, 2-1-1-1 Midorigaoka-Higashi, Asahikawa 078-8510; , 2: Department of Microbiology, School of Human Science and Environment, University of Hyogo, Himeji 670-0092; and , 3: Department of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo 060-0818, Japan,

Publication date: 2012-01-01

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