Impaired chemotaxis and cell adhesion due to decrease in several cell-surface receptors in cathepsin E-deficient macrophages
Source: The Journal of Biochemistry, Volume 145, Number 5, 27 May 2009 , pp. 565-573(9)
Publisher: Oxford University Press
Abstract:Cathepsin E is an endo-lysosomal aspartic proteinase exclusively present in immune system cells. Previous studies have shown that cathepsin E-deficient (CatE-/-) mice display aberrant immune responses such as atopic dermatitis and higher susceptibility to bacterial infection. However, the mechanisms underlying abnormal immune responses induced by cathepsin E deficiency are still unclear. In this study, we found that the cell-surface levels of chemotactic receptors, including chemokine receptor (CCR)-2 and N-formyl peptide receptors (FPRs), were clearly diminished in CatE-/-macrophages compared with those in wild-type cells. Consistently, chemotaxis of CatE-/-macrophages to MCP-1 and N-formyl-methionyl-leucyl-phenylalanine was also decreased. Similar to the chemotactic receptors, the surface expressions of the adhesion receptors CD18 (integrin β2) and CD 29 (integrin β1) in CatE-/- macrophages were significantly decreased, thereby reducing cell attachment of CatE-/- macrophages. These results indicate that the defects in chemotaxis and cell adhesion are likely to be involved in the imperfect function of CatE-/-macrophages.
Document Type: Regular paper
Affiliations: 1: Department of Dental Pharmacology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki 852-8588; , 2: Department of Fixed Prosthodontics, Graduate School of Dental Science, Kyushu University, Fukuoka 812-8582; , 3: Department of Biochemistry, Daiichi University College of Pharmaceutical Sciences, Fukuoka 815-8511; , 4: Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, 061-0293; , 5: Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582; , 6: Pharmacology, Graduate School of Dental Science, Kyushu University, Fukuoka 812-8582; and , 7: Proteolysis Research Laboratory, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan,
Publication date: 2009-05-27