NGR Enhanced the Anti-Angiogenic Activity of tum-5

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By this author: Meng, Jieru ;&nbsp; Ma, Nan ;&nbsp; Yan, Zhen ;&nbsp; Han, Wei ;&nbsp; Zhang, Yingqi

Authors: Meng, Jieru; Ma, Nan; Yan, Zhen; Han, Wei; Zhang, Yingqi

Source: The Journal of Biochemistry, Volume 140, Number 2, 15 August 2006 , pp. 299-304(6)

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Abstract:

Tumstatin is an angiogenesis inhibitor. The anti-angiogenic activity of tumstatin is localized to the 54-132 amino acids. NGR motif is a marker of angiogenic endothelial cells. We synthesized the gene fragment encoding the amino acids 45-132 of tumstatin (tum-5) and coupled a NGR (CNGRCVSGCAGRC) motif to the C-terminal of tum-5 (tum-5-NGR). The both were inserted into pQE30 expression vector and expressed in Escherichia coli. The anti-angiogenic effects of tum-5-NGR and tum-5 were examined in vivo. The results demonstrated the effect of the former was more significant than the latter. After S180 murine cancer xenografts in BALB/c mice were treated with tum-5-NGR or tum-5, tum-5-NGR displayed more significant tumor growth inhibition than tum-5. Binding of tum-5-NGR to normal and tumor tissues was also evaluated. The results showed that the accumulation of tum-5-NGR in tumor tissue was much more than in normal tissues. These data suggest that NGR enhance the anti-angiogenic activity of tum-5.

Document Type: Research article

DOI: http://dx.doi.org/10.1093/jb/mvj152

Publication date: 2006-08-15